Diana
Published in Bloodo Blog · 5 min read · May 7, 2024
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The Basics of Our Test
The Bloodo™ NAD Test uses an advanced technique to measure your total NAD levels, which include both NAD+ and NADH. This measurement is crucial because it reflects your cellular health and energy status.
Technical Details Made Simple
Method: We use the enzymatic cyclic assay, a highly accurate method, to detect total NAD.
Suitability: This test is designed for analyzing dried blood spots, making it convenient for home use.
Precision: With a coefficient of variation at 3.3%, our test is highly reliable.
Figure 1. Typical calibration curve of Bloodo NAD Test. NAD concentrations are given accounting for a dilution and shows total NAD concentration in human blood.
Understanding the Numbers
Limit of Detection: We can detect NAD levels as low as 2.7 µmol/L in your blood.
Linear Range: Our test accurately measures NAD levels in the range of 8–100 µmol/L.
Figure 2. Evaluation of the Bloodo NAD Test reproducibility. NAD concentrations are given accounting for a dilution and represent total NAD concentration in human blood.
Easy Sample Handling
Shipping and Storage: Both the test kits and the collected samples can be shipped and stored at room temperature, simplifying the process.
Diving into the Research and Data
Calibration and Accuracy: We use a calibration curve against the NAD standard to ensure accuracy. This means we can precisely measure your NAD within the identified range.
Consistency in Testing: We’ve tested over 100 samples to ensure the test’s reproducibility. Most samples showed very little difference between duplicate measurements.
Figure 3. Bloodo NAD Test results of testing using DBS samples from volunteers taking NAD supplements.
Real-World Application
NAD Supplement Monitoring: We monitored NAD levels in volunteers taking NAD supplements over several days. This study showed significant changes in NAD levels, highlighting our test’s ability to track real changes in your body.
Figure 4. Control testing of BloodoTM NAD Test performed using typical DBS samples (red) and DBS samples with removed NAD (yellow). Error bars represent ± 2 standard deviations from three independent measurements.
Control Group: For comparison, a control group taking nicotinamide showed different patterns, underscoring our test’s sensitivity to NAD-specific changes.
Control Testing for Accuracy: We’ve rigorously tested the Bloodo™ NAD Test to ensure it exclusively measures NAD. This included tests with and without the removal of NAD from samples, confirming the specificity and reliability of our method.
Figure 5. Control testing of Bloodo NAD Test using DBS samples (red), NAD standards (yellow), and DBS samples with added NAD standards (dark red). Error bars represent ± 2 standard deviations from three independent measurements.
The Takeaway
The Bloodo™ NAD Test is more than just a simple health test; it’s a window into understanding your body’s cellular vitality. With proven accuracy, consistency, and ease of use, it’s a powerful tool in your health and wellness journey.
Disclaimer While this guide simplifies the technical aspects of our test, we recommend consulting with healthcare professionals for a comprehensive understanding of your NAD levels and overall health.
1. Braidy N, Berg ,J Clement ,J Khorshidi F, Poljak A, Jayasena T, Grant R, Sachdev P. Role of Nicotinamide Adenine Dinucleotide and Related Precursors as Therapeutic Targets for Age-Related Degenerative Diseases: Rationale, Biochemistry, Pharmacokinetics, and Outcomes. Antioxid Redox Signal. 2019 Jan 10;30(2):251-294. doi: 10.1089/ars.2017.7269. Epub 2018 May 1. PMID: 29634344; PMCID: PMC6277084.
2. Fang ,J Chen W, Hou P, Liu Z, Zuo M, uLi S, Feng ,C Han Y, Li P, Shi ,YShao .C NAD+ metabolismbased immunoregulation and therapeutic potential. Cell Biosci. 2023 May 10;13(1):81. doi: 10.1186/513578-023-01031-5. PMID: 37165408; PMCID: PMC10171153.
3. Li W, Sauve A. NAD+ content and its role ni mitochondria. Methods Mol Biol. 2015;1241:39-48. doi: 10.1007/978-1-4939-1875-1_4. PMID: 25308486.
4. Wilson N, Kataura ,TKorsgen ME, Sun C, Sarkar S, Korolchuk V.l The autophagy-NAD axis ni longevity and disease. Trends Cell Biol. 2023 Sep;33(9):788-802. doi: 10.1016/j.tcb.2023.02.004. Epub 2023 Mar 5. PMID: 36878731.
Bloodo™ NAD Test: Specifications
The Basics of Our Test
The Bloodo™ NAD Test uses an advanced technique to measure your total NAD levels, which include both NAD+ and NADH. This measurement is crucial because it reflects your cellular health and energy status.Technical Details Made Simple
Method: We use the enzymatic cyclic assay, a highly accurate method, to detect total NAD.
Suitability: This test is designed for analyzing dried blood spots, making it convenient for home use.
Precision: With a coefficient of variation at 3.3%, our test is highly reliable.
Understanding the Numbers
Limit of Detection: We can detect NAD levels as low as 2.7 µmol/L in your blood.
Linear Range: Our test accurately measures NAD levels in the range of 8–100 µmol/L.
Easy Sample Handling
Shipping and Storage: Both the test kits and the collected samples can be shipped and stored at room temperature, simplifying the process.
Diving into the Research and Data
Calibration and Accuracy: We use a calibration curve against the NAD standard to ensure accuracy. This means we can precisely measure your NAD within the identified range.
Consistency in Testing: We’ve tested over 100 samples to ensure the test’s reproducibility. Most samples showed very little difference between duplicate measurements.
Real-World Application
NAD Supplement Monitoring: We monitored NAD levels in volunteers taking NAD supplements over several days. This study showed significant changes in NAD levels, highlighting our test’s ability to track real changes in your body.
Control Group: For comparison, a control group taking nicotinamide showed different patterns, underscoring our test’s sensitivity to NAD-specific changes.
Control Testing for Accuracy: We’ve rigorously tested the Bloodo™ NAD Test to ensure it exclusively measures NAD. This included tests with and without the removal of NAD from samples, confirming the specificity and reliability of our method.
The Takeaway
The Bloodo™ NAD Test is more than just a simple health test; it’s a window into understanding your body’s cellular vitality. With proven accuracy, consistency, and ease of use, it’s a powerful tool in your health and wellness journey.
Disclaimer
While this guide simplifies the technical aspects of our test, we recommend consulting with healthcare professionals for a comprehensive understanding of your NAD levels and overall health.
1. Braidy N, Berg ,J Clement ,J Khorshidi F, Poljak A, Jayasena T, Grant R, Sachdev P. Role of Nicotinamide Adenine Dinucleotide and Related Precursors as Therapeutic Targets for Age-Related Degenerative Diseases: Rationale, Biochemistry, Pharmacokinetics, and Outcomes. Antioxid Redox Signal. 2019 Jan 10;30(2):251-294. doi: 10.1089/ars.2017.7269. Epub 2018 May 1. PMID: 29634344; PMCID: PMC6277084.
2. Fang ,J Chen W, Hou P, Liu Z, Zuo M, uLi S, Feng ,C Han Y, Li P, Shi ,YShao .C NAD+ metabolismbased immunoregulation and therapeutic potential. Cell Biosci. 2023 May 10;13(1):81. doi: 10.1186/513578-023-01031-5. PMID: 37165408; PMCID: PMC10171153.
3. Li W, Sauve A. NAD+ content and its role ni mitochondria. Methods Mol Biol. 2015;1241:39-48. doi: 10.1007/978-1-4939-1875-1_4. PMID: 25308486.
4. Wilson N, Kataura ,TKorsgen ME, Sun C, Sarkar S, Korolchuk V.l The autophagy-NAD axis ni longevity and disease. Trends Cell Biol. 2023 Sep;33(9):788-802. doi: 10.1016/j.tcb.2023.02.004. Epub 2023 Mar 5. PMID: 36878731.